Original Articles: 2015 Vol: 7 Issue: 6
Co-expression of quinicacidbiosynthesis key gene in Escherichia coli
Quinic acid is one of small important molecular compound which belong to aromaticity, and it is widely used in the field of foodstuff and chemicalÃ¯Â¼ÂŒas well as medicine. Genic project bacteria which product quinic acid is constructed Using the metabolism project technique. new metabolic quinic acid is synthesized through colishikimic acid way of coliform. Using the metabolism project tradition research strategy, the content of enzyme is enhanced through increasing copy of essential enzyme gene, as well as relieving the feedback inhibition which the essential enzyme receives to maintain activity of enzyme, then the distribution of microorganism's metabolism flows is adjusted. The goal is flowing the carbon metabolism to the greatest degree of producting of quinic acid.