In this present study Streptomyces was isolated fro m the Mangroves soil and identified by gram stainin g, biochemical tests and microscope observation. The o ptimum growth condition for the isolated organisms for the α - amylase production were such as pH, temperature, su bstrate concentration. On the production α - amylase using the isolated organisms the four different substrate suc h as under varying condition of coconut oil cake, g roundnut oil cake, wheat bran, rice bran,were assessed coconut o il cake was produced the maximum amount of α -amylase in, pH4 (18.5 ± 0.02) temperature25 0 C ( 8.5 ± 0.02 ) substrate concentration 15g (12.5 ± 0.02) . Thus from the above study, it is concluded that coconut oil cake produces higher amount of α - amylase compared to groundnut oil cake, wheat bran, ricebran. Thin layer chromatographic the end products librated by to action of α -amylase to separate the glucose and maltose by using thin layer chromatogra phic.