Brucellosis, a bacterial zoonotic disease, primarily of cattle and is prevalent worldwide. The disease results in abortion and/or infertility in affected animals and undulant fever in human. It is an important cause of economical losses and human suffering. This disease is spread worldwide with areas of high endemicity in the Mediterranean, India, Middle East, South and Central America and Asia. The disease is caused by organisms belong to the genus Brucella. The true incidence of human brucellosis however, is unknown in most of the countries including India. In animals, the disease is usually asymptomatic in nonpregnant females, while pregnant females develop a necrotic placentitis and ulcerative endometritis, usually resulting in abortion. Many serological tests have been developed for the diagnosis of brucellosis. The most commonly used tests are standard tube agglutination test (STAT), the coombs anti-brucella test, the rose bengal plate agglutination test (RBPT), complement fixation test (CFT) and indirect haemolysis test (IHLT). The antigenic similarity of Brucella with other gram-negative organisms gives the false positive reactions in serodiagnosis, which reduces the specificity for diagnosis. Keeping in view the importance of brucellosis as an emerging infection and its prevalence in our country, there is a definite need to develop a specific diagnostic assay for Brucella infection. In this study, two diagnostically important recombinant proteins were cloned, expressed, purified and characterized, which can be used as a diagnostic antigen.