Gingivitis is the inflammation of gingiva and a milder form of periodontal disease. Studies have shown that Vitamin D supplementation may reduce susceptibility to gingival inflammation through its anti-inflammatory effect by inhibiting antigen induced T cell proliferation and cytokine production. The aim of the study is to clinically evaluate the effect of vitamin D supplementation on the outcomes of non- surgical periodontal therapy in post-menopausal women with gingivitis. Total of 20 post-menopausal women visiting the department of Periodontology, D.A.P.M.R.V Dental College, were included in the study, with gingivitis (GI score >1). Out of which 10 were on vitamin D supplements (>400 IU/day) since one year and 10 subjects were not on any vitamin D supplements. Gingival, plaque and bleeding indices were recorded at baseline, 1, 2 and 3 months interval post scaling. On statistical analysis, there was significant improvement in all the three clinical parameters within the groups at different intervals (P<0.05) but however the difference in improvement (P>0.05) was not statistically significant between the groups.