There is increasing interest in herbal medicine and natural antioxidant products for use as medicines and food additives. The present work was identified the chemical composition compounds of Vitex pseudo-negundo leaves essential oil. This experimental study, which carried out in 2013 in Lorestan Medical Sciences University. Essential oil of Vitex leaves was prepared. The components of Vitex pseudo-negundo leaves essential oil were analyzed with gas chromatography/mass spectrometry (GC/MS). GC/MS data and retention indices for reference essential oil leaves samples were used to identify 30 constituents. These compounds make up a total of 99.00 percent essential oil. 2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene; 1,8-Cineole and Phenol, bis(1,1-dimethylethyl are the most of compounds of Vitex pseudo-negundo leaves essential oil. This study showed that essential oil of Vitex pseudo-negundo is a source easily accessible of natural antioxidants such as 1, 8-Cineole and Sabinene and it may be suitable for use in food and pharmaceutical applications.