Cassia occidentalis L. (Leguminacea), commonly known as ‘Coffee Senna’, is used in Unani medicine for liver ailments and is an important ingredient of several polyherbal formulations marketed for liver diseases. The aqueous extract was chemically examined and pharmacologically screened for Hepatoprotective activity. The structure of the new compound isolated from the extract and elucidated by using Modern spectral and chemical techniques. And was found to be an anthraquinone glycoside. The hepatoprotective effect of aqueous extract of seeds of Cassia occidentalis was studied on rat liver damage induced by paracetamol and by monitoring SGPT, SGOT, alkaline phosphate, total bilirubin, and histopathological alterations. The extract of seeds of plant produced significant hepatoprotection.