Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 4

Characterization of Caffeine Isolated from Camellia Sinensis Leaves of Sikkim Himalayan Region


The present study was conducted to isolate the caffeine from the Camellia sinensis (green tea) leaves obtained from the Sikkim Himalayan Region followed by its spectroscopic and HPLC studies. Caffeine (3,7-Dihydro-1,3,7-trimethyl-1H-purine-2,6-dione) was isolated from leaves using a liquid-liquid extraction method. Isolated product was evaluated by using TLC, UVVisual Spectrophotometer, FTIR, NMR studies and HPLC. The data obtained from these studies was compared with standard caffeine. From above studies, it was concluded that the compound isolated from Camellia sinensis leaves is caffeine and having the characteristics almost similar to that of standard caffeine.