In this research we evaluated the potential of antibiotic production and characterize the FTIR spectroscopy and HPLC (High performance liquid chromatography) analysis pattern of Streptomyces from various semi-arid locations of Jaipur, Rajasthan. Regarding this, five soil samples were collected randomly from three different green cover areas of Jaipur. Following the extraction of secondary metabolite, the HPLC and FTIR spectroscopy analysis was carried out for characterization of various extracts. Considering the coordinate analysis of HPLC and FTIR spectroscopy pattern, isolate A1 is producing Rifampin, isolate A2 might be potential producer of Capreomycin IA and Capreomycin IB while isolate A3 is producing Clavulanic acid. Besides A3 is also producing antifungal compounds. FTIR analysis showed peaks between 600-800 cm-1 showing Alkyl Halide, 1080-1360 cm-1 Amine group, 1400-1600 cm-1Aromatic group, 1670-1820 cm-1Carbonyl group and peaks between 1800-1830 cm-1 showing anhydride group. These results highlight the importance of Streptomyces isolates in antibiotic and antifungal production.