Paper mill effluent is a major source of pollution generating industry discarding huge amount of intensely colored effluent. Some are rich in wood fiber and harbor good carbon source further the primary and de-inking paper mill effluent others carry effluents rich in nitrogen and phosphorus. Lack of infrastructure, technical manpower research and development facilities restrict these mills to recover the chemicals. The chemical oxygen demand of the emanating stream is quite high and floating minuscule of debris. The intention of this research paper is to identify the predominant bacteria and fungi in paper mill effluent in addition of evaluating the degradation efficiency of individual isolates and combination of isolates to treat the released effluent. Effective floc formation and degradation was attained in Pseudomonas alkaligenes + Enterobacter spp. combination which enhance clearing and settling process in the treatment plants. Rapid increase of population and the increased demand for industrial establishments to meet human needs have created problems such as over exploitation of available resources, increased pollution in air and water environment hence there is a growing demand to treat the effluent with the native industrial samples isolates.