Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 5

Characterization and antioxidant activity of carotenoid mixtures present in Rhodococcus sp. and Gordonia sp

Abstract

Structure elucidation of carotenoids pigments present in Rhodococcus sp. and Gordonia sp. was performed with Fourier-transformed infrared spectrometry (FT-IR), high performance liquid chromatography (HPLC), ultravioletvisible spectrometry (UV-Vis), electrospray ion-mass spectrometry (ESI-MS) and proton and carbon nuclear magnetic resonance (1H and 13C NMR). A HPLC carotenoid quantification method was developed, where antioxidant activity with 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical was performed to calculate the inhibitory concentration 50 (IC50) of each carotenoid mixture; contribution of each carotenoid present in mixtures and possible interactions were determined. Rhodococcus sp. and Gordonia sp. presented two and three carotenoids respectively; analytical techniques allowed to suggest that carotenoids present in these strains might have structures similar to the reduced form of 4-oxo retinaldehyde, 8’-apoastaxanthinal and astaxanthin dirhamnoside. Rhodococcus sp., Gordonia sp. and β-carotene presented an IC50 of 1.07, 0.09 y 19.49 μg/mL respectively. Possible interaction in Gordonia sp. between the reduced form of 4-oxoretinaldehyde like carotenoid and astaxanthin dirhamnoside like carotenoid was assessed.