Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2012 Vol: 4 Issue: 4

Central composite design application for optimization of aqueous two-phase extraction of protein from shrimp waste


An experimental design was used to optimize protein partitioning from shrimp waste using Polyethylene glycol/sodium citrate aqueous two-phase systems (ATPS). Four factors like PEG’s molecular weight (A), pH (B), Addition of sodium chloride (C), and Tie Line Length (D) affecting the protein partitioning were studied. To build the mathematical model and minimize the number of experiments for the design parameters, response surface methodology (RSM) with a central composite design was defined based on the conditions found for the highest partitioning by preliminary tests. The best conditions of partioning were achieved using ATPS composed of PEG 4000, sodium citrate salt, pH8, 1M Addition of sodium chloride, and 40 Tie Line Length. The maximum percentage yield of protein extracted from shrimp waste was about 90.54%. Analysis of response surface plots as a function of, PEG molecular weight, pH, and concentration of sodium chloride, and Tie Line Length was studied.