The study was designed to scientifically evaluate the cardio protective effect of ethanolic extract of Nelumbo nucifera rhizomes, a traditional medicinal herb, on isoproterinol-induced myocardial necrosis in rat model. The albino male wistar rats were divided into five different groups with six animals in each group. Group I serves as a control group, group II were given isoproterenol (85 mg/kg s.c) for 2 days while Group III was treated with metoprolol (10 mg/kg) for 30 days and isoproterinol on last 2 days. This group serves as standard group and its effect was compared with that of test drug (Nelumbo nucifera extract). Group IV and V were given Nelumbo nucifera rhizomes extract (300 mg/kg and 150 mg/kg respectively) for 30 days and isoproterinol on 29th and 30th day. The level of enzymes Aspartate transferase (AST), Alanine transferase (ALT), Lactate dehydrogenase (LDH), Cretinine kinase (CK) were estimated in serum and the extent of necrosis was studied by grading. Isoproterenol significantly increased the activities of AST, ALT, CK, LDH in serum. Pretreatment with the ethanolic extract of Nelumbo nucifera had a significant decrease in the level of marker enzymes. The effect was compared with the effect on serum marker enzymes in the group treated with Metaprolol. The metaprolol treated group has shown reduced level of marker enzymes, nearer to the control group. It was found to be more protective at dose 300mg/kg as compared to 150 mg/kg dose.