Silver in its original form or in the form nanoparticles has been known from time immemorial in order to control different types of diseases. During last few decades, bacteria have made themselves resistant to varied antibiotics available in the market based on their genetic configurations. In our present study, simple and effective approach was performed to synthesize potent silver nanoparticles (AgNPs) from hyphomycetes fungus, Alternaria using potato dextrose broth with AgNo3. The appearance of yellowish brown color in the conical flask suggested the formation of AgNPs. The supernatant of the fungus culture changed the solution into yellowish brown color upon the completion of 10 minute reaction. The characterization and silver nature of silver nanoparticles was confirmed by Uv-Vis spectrophotometer, Field emission scanning electron microscopy (FESEM) and XRD analysis. Size of the nanoparticles measured between 50nm to 60nm by FESEM. AgNPs showed impressive antimicrobial activity against the selected pathogens, but when the nanoparticles were tested against the test pathogens combined with two drugs, penicillin and tetracycline, the efficacy of the drugs was multiplied, particularly in the case of tetracycline in comparison to penicillin.