The present study reports the sorption capacity of mushroom Pleurotus eous which is evaluated on the biosorption of heavy metals such as lead, chromium and nickel from aqueous solutions. The optimum sorption conditions were studied for each metal separately. The desired pH of the aqueous solution was found to be 5.0, 3.0 and 7.0 for the removal of lead (Pb), chromium (Cr) and nickel (Ni) respectively. The percentage removal of all the heavy metals was found to be biosorbent dependent and found to increase with the increase in biosorbent dosage and agitation speed. The contact time was optimized to be 9 hours for all the three metals. Comparative studies on biosorption of three heavy metals Pb, Cr, Ni by the mushroom P.eous shows high metal uptake with respect to lead rather than chromium and nickel. Hence the present data demonstrates the suitability of fruiting bodies of P.eous as an efficient biosorbent for the removal of toxic heavy metals and further the studies confirms that the biosorption varies with metals.