Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2013 Vol: 5 Issue: 7

Bio-degradation of harmful textile dyes by marine bacteria from Tuticorin coastal Waters Southeastern India

Abstract

The minimum and maximum level of dye degrading bacterial density was found to be 2.1 X 103 CFU/g and 8.5 X 103 CFU/ respectively. The strains were tested for their degradation efficiency using 6 dyes namely Malachite green, Majenta MB, Turquoise blue H, Orange Fast Boardeux r GB, Direct blue 2B and Direct Congo Red. Among the 5 strains, MSB 4 exhibited maximum degradations activity against all the six dyes (Malachite green – 6 mm, Majenta MB, Turquoise blue Ea, Orange fast boardeux – 4 mm and Direct blue 2B, Direct Congo Red – 2mm). The active strain MSB4 was used for qualitative estimation of degradation of dyes at different concentrations. The percentage of dye discoloration of dye was observed against Majanta MB (68.25%) followed by orange Fast boardeux (64.10%), Congo red (63.55%), Malachite gren (61.89%) and Direct blue (22.09%), while lowest discoloration was observed against Turquoise blue (12.06%) at 100ppm concentration. At 500 ppm concentration the maximum percentage of dye discoloration was observed against direct blue (9.03%). The active strain MSB4 was further identified as Pseudomonas sp based on the morphological and biochemical tests. This work concludes that the Pseudomonas sp isolated from mangrove sediment have the ability to degrade harmful dyes such as Direct blue, Direct Congo Red, Turquoise blue H, Orange fast boardeux GB and Malachite green which could be considered for the bio-degradation of colour effluent discharged from paper and printing industries. Further works has to be done to optimize the discoloration effect this bacterium.