This paper reports the details of experimental investigations carried out on the Kappaphycus sp., a family of red algae. Chemical constituents such as carbohydrates, lipids, proteins, fat, amino acids, fatty acids, phenols, sterols were estimated from Kappaphycus sp. Sample was collected from the sea coast of Rameshwaram, Tamil Nadu, India in the form of wet and living sample. Standard material methods have been followed for estimation of chemical constituents available in Kappaphycus sp. From the experimental studies, it is observed that the carbohydrate available in Kappaphycus alvarezii is 2.67 gm/100gm. The total lipid content is 1.09gm/100gm. The total protein content is very high about 18.78gms/100gm compared to all other substances. Fat content in the species is 1.09gm/100gm. Total phenol content is 4.565gm/100mg. Further, it can be noted that the phenolic content is very much less than proteins but more than carbohydrates, lipids and fats.