Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 4

Biochemical analysis of in vitro derived plants of Sterculia urens during acclimatization


Sterculia urens is a commercially important tree with wide applications. Nodal explants were grown on MS medium supplemented with various growth regulators like 6-benzyladenine (BA), Thidiazuron (TDZ) and Kinetin (Kn). These shoots were subsequently rooted in vitro on half strength MS medium containing various concentrations of auxins like Indole-3-butyric acid (IBA) and Naphthalene acetic acid (NAA). Plants were acclimatized to ex vitro conditions and established in the field. Role of peroxidase, poly phenol oxidase, total proteins, total carbohydrates and total phenols were studied during acclimatization by estimating their at an interval of 20, 40 and 60 days. Peroxidase and poly phenol oxidase level decreases from 20 to 60 days where as levels of total proteins, carbohydrates and phenols increases