Biofuels in particular are receiving climbing attention due to the increase in industrialization, population, global demand for energy driven by fast depletion of conventional fuels. Biobutanol a solvent is a convertible to jet fuel blends with gasoline and also acts as a rich fuel extender. Biobutanol when blended with fuel can be used in internal combustion engines releasing carbon dioxide making environmental friendly biofuel. Further biobutanol is used much than ethanol due to its high calorific value, less volatile, low vapor pressure, relatively low heat of vaporization and is highly explosive over bioethanol. As well biobutanol rather than synthetic butanol is produced using ABE (Acetone Butanol Ethanol) fermentation reducing the use of conventional chemical methods. ABE fermentation was achieved using Clostridium species whose genes help in the conversion of biomass into butanol. In addition to this, yield and productivity of the biofuel can be used to recover high yields biobutanol through metabolic and genetic manipulation and by inculcating efficient pre-treatment technologies. In this article higher yields (0.57- 0.65 %) of biobutanol is produced using lignocellulosic substrate,Moringaolieferaa soft wood whose bark through proper pretreatment and saccharification process yields monosacharideswhich are fermented using Clostridium sp..(MTCC 1349) for the maximum production ofbiobutanol.