Simple high-Performance Liquid Chromatographic method for determination of Nevirapine in human plasma is hereby reported. Carbamazepine was used as internal standard (IS). The internal standard and the drug were extracted into basified di-isopropyl ether, dried with a current of air using hair drier. The dried sample was reconstituted with 120 µl of mobile phase followed by injection of 90 µl of the reconstituted sample into the chromatograph. A reversed phase column C18 was used and the mobile phase consisted of 250ml acetonitrile and 800 ml phosphate Buffer (pH 7.5). Detection was achieved at 282nm and both NVP and IS were well separated from endogenous substances. The method involves single step extraction procedure and applies a commonly available internal standard. It is suitable for use in laboratories of developing countries as it applies readily available carbamazepine as internal standard, C-18 column and does not require nitrogen evaporation system and high speed centrifuges whose availability may be an issue in resource constrained laboratories.