Cyanobacteria inhabit a range of diverse and extreme habitats and have potential to produce an elaborate array of secondary metabolites with unusual structures and potent bioactivity. The concept of biological control for the health maintenance has received widespread attention during the last few years. Therefore, the main project of this investigation focuses for active phytochemical substances that could be used as antioxidants, antimicrobials agents, anticancer and cholesterol-lowering agents. To achieves this target, four proceeding treatments (soaking, autoclaving, NaCl 10% and soxhlet) and four different solvents (Methanol, acetone, chloroform and petroleum ether) used for preparation of Spirulina platensis extracts. The common major secondary compounds like, carbohydrate, lipid, phenol, flavonoids and tannins were extracted using four polar and nonpolar solvents. Of the four solvents tested, acetone was the best solvent for isolation of antimicrobial compounds, anticancer agents, antioxidants compounds and cholesterol-lowering agents from the tested algae followed by methanol. The soaking treatment was the best method for extraction the active compounds followed by autoclaving. The present study elaborates the bioactive content of Spirulina platensis during gas chromatography mass spectrometry (GC-MS) analysis.