The evaluation of antimicrobial activity has been the subject of research, mainly due to the presence of bacterial multidrug resistance of conventional treatments. This research aimed to investigate the antimicrobial and cytotoxic activity of ethanolic crude extracts from leaves, leaf sheath, and stem of Cocos nucifera Linn (green dwarf coconut tree) in vitro. The antimicrobial activity was evaluated against gram-positive bacteria S. aureus and Gram-negative bacterias E. coli and P. aeruginosa and the Candica albicans fungus, through the methods of agar diffusion and identification of minimum inhibitory concentration through microdilution. The cell viability assay was performed using the J774 cell line by MTT. The sheath and leaf extracts of C. nucifera L. inhibited bacterial growth of S. aureus and P. aeruginosa with the minimum inhibitory concentration of growth of 10 mg (5%), and there was no inhibition of E. coli and C. albicans growth at a concentration of 1.5 x 106 CFU by the extracts at 10%. The extracts showed no cytotoxicity at the concentrations of 200 and 100 μg/mL; the ethanolic extract from sheath did not exhibit cytotoxicity at 1000 μg/mL. The results show partial antimicrobial and cytotoxicity activities.