Anxiolytic disorders have high prevalence in society and many medications such as benzodiazepines and serotonin inhibitors are used to treat anxiolytic disorders nowadays. To overcome the side effects of synthetic drugs, further research needs to be conducted on natural ingredients. This study was conducted to determine the effect of aqueous extract of jujube on sleep duration and anxiety level of mice. In this experimental study, 80 male albino mice weighing 30 to 25 grams were used. They were randomly assigned to eight groups (6 experimental groups and two control groups, each group contains10 mice). To assess sleep period, behavioral method (Angel) was used and animals were divided into three experimental groups and one control group. To assess anxiety level, animals were divided into three experimental groups and one control group, and elevated plus maze was used in this regard. Standard indices of anxiety assessment (the number of entry and time spent in open arm) were examined and recorded for 5 minutes by observing them. Three doses of aqueous extract of Jujube (200-400-600 mg / kg)were injected to experimental groups, and saline was injected intraperitoneally in control group. the results showed that the aqueous extract of jujube at dose of 600 increases sleep period considerably compared with control group. (P <0.05). In addition,400 and 600 doses of aqueous extract of jujube significantly increased the number of entry (p <0.05) and time spent (p <0.05) in elevated plus maze open arm compared with control group. This study showed that aqueous extracts of jujube increases sleep period and reduces anxiety levels in mice