Antithrombotic, anticoagulant properties of medicinally used Desmodium gyrans (DC)was studied using 70% methanolic extract. Clotting time, prothrombin time, plasma recalcification time and platelet aggregation studies were performed in plasma collected from Desmodium gyrans extract treated male Sprague Dawley rats. Inhibition of lipid peroxidation was assessed in vitro using platelet rich plasma sample. The treatment with extract gave a delay in coagulation by 14.6% in prothrombin time assay. Plasma recalcification time also was enhanced with treatment. Collagen induced platelet aggregation of platelet rich plasma was brought down significantly in dose dependent manner. On treatment lipid peroxidation was brought down by 29.94% compared to the control.