Eight flavonoids, quercetin (1), quercetin 3-O-β-D-glucopyranoside (2), quercetin3-O-α-L-rhamnopyranoside(3), myricetin (4), myricetin 3-O-β-D-galactopranoside (5), myricetin 3-O-α-L-rhamnopyranoside (6), desmanthin-1 (7), and naringenin (8), in addition to four phenolic acids, brevifolin carboxylic acid (9), ellagic acid (10) gallic acid (11), and methyl gallate (12) were isolated from the leaves of Acrocarpus fraxinifolius. The chemical structure of the isolated phenolic compounds were established by 1H, 13C-NMR, UV, and HR-ESI-MS. Ellagic acid produced an antiproliferative effect on the triple negative breast cancer cells MDA-MB-231 with IC50 of 38.7 μM. The aq. methanolic extract and most of the isolated compounds showed significant antioxidant activity in comparison with the positive control; rutin using TLC-DPPH˙-image processing procedures. Also the methanol extract and some isolated compounds exhibited antibacterial activity against P.aeruginosa L. subtilis E. coli S. aureus, it did not show any antifungal activity.