Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

header
Reach Us +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2019 Vol: 11 Issue: 12

Anti-oxidative and Anti-inflammatory Effects of Carissa spinarum L., Croton oblongifolius Roxb and Dioscorea bulbifera L. in LPS-Induced RAW264.7 Cell

Abstract

The aim of this study was to evaluate the anti-inflammatory effect for ethanolic extracts of Carissa spinarum L. (C. spinarum), Croton oblongifolius Roxb. (C. oblongifolius) and Dioscorea bulbifera L. (D. bulbifera) in lipopolysaccharide (LPS)-induced RAW264.7 cell and anti-oxidative activity of these extracts by 1-Diphenyl-2-picryl hydroxyl (DPPH)-assay, Nitric Oxide (NO)-assay and Super Oxide (SO)-assay. Cell viability was done by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)-assay. Anti-inflammatory potential was assessed by evaluating the ability of the tested extracts to inhibit NO generating from LPS-stimulated RAW264.7 cells. The present work investigated that tested extracts showed the strong anti-oxidative activity on different forms of free radicals such as DPPH, NO and SO. Among them, C. oblongifolius Roxb. showed stronger anti-oxidative effects on NO and SO radicals than the other extracts. MTT-assay revealed that these tested extracts have no cytotoxic effects on RAW264.7 cell for concentration at and below 250 μg/mL. According to cytotoxic result, (62.5-250) μg/mL (cell viability>85%) were selected to determine NO inhibition experiment. Tested extracts significantly decreased the production of NO in LPS-stimulated RAW264.7 cells in a concentration-dependent manner. IC50 values of NO inhibition (generated by LPS) of C. spinarum L., C. oblongifolius Roxb. and D. bulbifera L. were 135.9 ± 10.8, 130.4 ± 7.8 and 159.1 ± 12.8 μg/m respectively. According to our results, C. oblongifolius Roxb. has both radicals scavenging and anti-inflammatory effects.