Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2015 Vol: 7 Issue: 5

Antioxidant capacities, phenolic, flavonoid and carotenoid content of various polarities extracts from three organs of Sechium edule (Jacq.) Swartz

Abstract

The objectives of this research were to study antioxidant activities from various fruit extracts of three organs of S. edule using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and CUPRAC (Cupric ion Reducing Antioxidant Capacity) and correlation of their total flavonoid, phenolic and carotenoid content with IC50 of DPPH antioxidant activities and EC50 of CUPRAC capacities. Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant capacities were tested using DPPH and CUPRAC assays. Determination of total phenolic, flavonoid and carotenoid content was performed by spectrophotometer UV-visible and their correlation with IC50 of DPPH scavenging capacities and EC50 of CUPRAC capacities were analyzed by Pearson’s method. Ethyl acetate pedicel extract of S. edule (PD2) had the lowest IC50 of DPPH scavenging activity 1.3 μg/ml, while ethyl acetate fruit extract of S. edule (FR2) had the lowest EC50 of CUPRAC capacity 147 μg/ml. Ethyl acetate fruit extract of S. edule (FR2) had the highest total phenolic content (3.21 g GAE/100 g), ethyl acetate leaves extract of S. edule (LV2) had the highest total flavonoid content (11.64 g QE/100 g) and the highest total carotenoid content (12.73 g BE/100 g). There was negatively high correlation between total phenolic content in leaves extracts of S. edule with their IC50 of DPPH. The negative and high correlation between total phenolic, flavonoid and carotenoid content in fruit and pedicel extracts with their EC50 of CUPRAC capacities. The IC50 of DPPH scavenging activities of three organs of S. edule had no linear result with their EC50 of CUPRAC capacities.