3-Substituted Schiff bases of Quinazolne-2,4-diones which belongs to the N-containing heterocyclic compounds. The aim of the present study is to study pharmacological study of 3-Substituted Schiff bases of Quinazoline2,4-diones. In the current course of work the novel Schiff bases of Quinazolne-2,4-diones have been synthesized and evaluated for Anti-oxidant and In vitro anti-inflammatory study. 3-Substituted Schiff bases of Quinazoline 2,4-diones have been evaluated for Antioxidant and anti-inflammatory activity. Antioxidant activity was evaluated by Ferric reducing antioxidant power (FRAP), 1,1-DPPH(2,2-diphenyl-1-picrylhydrazyl) And anti-inflammatory activity by membrane stabilization method. The newly synthesized compound F and I showed highest anti-oxidant activity .This compound was further evaluated for in-vitro anti-inflammatory activity by membrane stabilization method. The result of the present study thus demonstrates the anti-inflammatory and anti-oxidant activity of newly synthesized 3-Substituted Schiff bases of Quinazolne-2, 4-diones.