Methanolic extract of Securinega leucopyrus flowers was evaluated for their in-vitro antimicrobial activity. The plant was collected from moist region of Wainganga River, Bhandara (Maharashtra), India. Securinega leucopyrus is extensively used as medicine in Indian folklore for treatment of various infectious diseases. The antimicrobial activity of the plant extracts against various strains of bacterial species was tested for the zone of inhibition using the disc-diffusion assay, minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) values. This extract showed profound inhibitory action on the growth of bacterial strains of some species of genera belonging to Acinetobacter, Bacillus, Brevundimonas, Brucella, Enterobacter, Escherichia, Micrococcus, Pseudomonas, Staphylococcus and Xanthomonas.