Phytochemical study and antimicrobial screening of Trigonella foenum graecum against diarrhoeal pathogens were carried out in this study. Diarrhoea causing bacteria Escherichia coli, Salmonella spp., Vibrio spp., and Shigella spp., was selected as test pathogens. Aqueous methanol and acetone extracts of Trigonella foenum graecum seeds were showed antimicrobial activity. Phytochemical study showed the presence of carbohydrate, alkaloid, saponins, tannins, phenolic compounds and flavonoids in methanol fraction of Trigonella foenum graecum seeds.