Thiosemicarbazone derivative at 1 and 2 position of the Biologically important Lawsone ( 2- hydroxy-1,4-naphthalenedione ) have been synthesized. This possesses a powerful chelating ability, appreciable analytical utility and significant biological activity. Metal complexes of TSCND with Ni(II) metal have been synthesized and studied with the help of spectral analysis and elemental analysis. The antimicrobial activity of the ligands and their metal complexes against bacteria and fungus has been carried out. It is found that the metal complexes have higher activities than those of free ligands