Cobaloximes of the type [Co(dmgH)2(B)Cl] ; where, dmgH- = dimethyl glyoximate, B= 3- chlorpyridine (3-PyCl), 3-bromopyridine (3-PyBr), 4-aminopyridine (4-AP), 4-methylpyridine (4-MP), 4-N,N’-dimethyl aminopyridine (4-DMAP) and 4-cyanopyridine (4-PyCN) were prepared, characterized by the usual spectroscopic techniques and screened against microorganism for antimicrobial activity with ciprofloxacin as standard. The cobaloximes were found to be active against most of the microbes. The cobaloxime containing 4-cyanopyridine as the axial ligand showed potential inhibition; whereas, the complex [Co(dmgH)2(3-PyCl)Cl] showed the least microbial growth.