In India, a large number of medicinal plants occur in the wild state. Presences of the phytochemical constituents such as alkaloids, flavonoids, tannin, and phenolics compounds have been reported to be important compounds in many other medicinal plants. These compounds exhibit diverse actions such antibacterial, analgesic, antifungal .antidiabetic and antioxidant etc. The main objective of this work was to study the antibacterial activity of methanolic extracts of Neem (Azadirachta indica), Rosemary (Rosmarinus officinalis) and Bottle guard (Lagenaria siceraria) leaves on some important Pathogenic organisms. The methanolic extracts of leaves of Neem, Rosemary and Bottle guard were evaluated for its antibacterial activity against staphylococcus aureus pseudomonas aeruginosa. , E.coli and Klebsiella using Agar well diffusion technique. The methanolic extracts of leaves of Neem, Rosemary and Bottle guard (MEAIL, MERML and MELSL) showed zones of inhibition on agar plates. The methanol leaf extracts of Neem leaves, Rosemary leaves and Bottle guard fruit showed significant antibacterial activity against Escherichia coli, Pseudomonas, Staphylococcus aureus and Klebsiella. Extensive studies may yield cost effective antibiotics for future