Plants have been a valuable source of natural products for maintaining human health. Plants are used in traditional medicinal systems have proved to be reliable sources of antimicrobial compounds. Medicinal plants have been considered interesting since they are frequently used in medicine as remedies for many infectious diseases. The plant Tephrosia purpurea has been used in different system of traditional medication for the treatment of diseases and ailments of human beings. It is reported to contain various flavonoides, alkaloids, steroids, phenolic compounds. It has been reported as anticancer, antioxidant, anti-inflammatory, antiulcer, hepatoprotective, immunomodulatory, antilithiatic, free radical scavenging, antileishmanial, antibiotic, antimicrobial, anyihyperglycemic, antilipidperoxidative and wound healing activities. The whole plant and its roots are used for medicinal purposes. The herb is useful both, internally as well as externally. In this study ethanol and acetone leaf & root extract of Tephrosia purpurea were investigated for in vitro antibacterial property by agar disc diffusion method. The crude extract of Tephrosia purpurea, the acetone and ethanol root extract showed good antimicrobial activity against the Salmonella typhi & Proteus mirabilis. Leaf extract of ethanol showed good antimicrobial activity against the Proteus mirabilis. Leaf extract of acetone showed moderate inhibition activity against the Staphylococcus aureus.