Ethanol, chloroform, methanol, acetone, ethyl acetate, hexane, petroleum ether and aqueous extracts of Parthenium hysterophorus from vellore were tested in vitro for their antifungal activities against, Aspergillus niger, Candidia albicans and Candida kefyr with the disc diffusion method. Methanol was the best solution for extracting the effective antifungal materials from the Parthenium hysterophorus used in this experimental and compared with standard drug, Ketoconazole. In the present study show the importance of in producing new bioactivity compounds having antifungal activity.