Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 10

Antifungal Activity and Association Study of (R)-(+)-citronellal Enantiomer with Amphotericin B, Fluconazole, Itraconazole and Miconazole against C. tropicalis Isolated from Vulvovaginal Secretions

Abstract

Vulvovaginal candidiasis (VVC) is among the most common diagnosis in women who seek gynecological services. The C. albicans is the main etiological agent. However, nowadays can be observed an increase of the prevalence of non-albicans species, such as C. tropicalis. Assess the antifungal potential of the (R)-(+)-citronellal [(R)-(+)-CT] isolated and associated to amphotericin B, fluconazole, itraconazole and miconazole, against C. tropicalis from vulvovaginal secretions. The enantiomer was solubilized in tween 80 and DMSO, posteriorly diluted in sterile distilled water up to the concentration of 2048μg/mL. The minimum inhibitory concentration (MIC) of the product was determined by microdilution in RPMI-1640, obtaining dilutions of 1024-4μg/mL. The minimum fungicidal concentration (MFC) was determined by the sabouraud dextrose agar (SDA) depletion technique from aliquots of 10μL of the MIC, MIC × 2 and MIC × 4. The antifungal susceptibility testing and the interfering effects of the association of the enantiomer with the standard drugs, were determined by disk-diffusion in SDA. The MIC of (R)-(+)-CT was 16μg/mL and the MFC 32μg/mL. A high resistance of the strands C. tropicalis to amphotericin B, itraconazole and miconazole was observed. The combination test of the enantiomer with the amphotericin B, as well as with the itraconazole, resulted in synergism 2 (66.6%) of the yeasts, and in association with the fluconazole 1 (33.3%) and miconazole 3 (100%) of synergic effect. The (R)-(+)-CT alone is fungicide for the 3 fungal strains and in association with the four antifungals increased the inhibition zones, increasing the sensitivity.