Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2011 Vol: 3 Issue: 1

Antibacterial studies on in vitro derived calli of Ocimum basilicum L.


The present study was aimed to screen the antibacterial potential of calli derived from internodal and leaf segments of Ocimum basilicum. Highest percentage of callus formation (internodal segments 75.2 ± 0.90; leaves segments 78.7 ± 0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3% sucrose and 1.0 mg/l of 2, 4 - Dichlorophenoxy acetic acid. Different types of calli were obtained of which, the friable, semi friable and creamy white coloured showed high proliferation rate. Five different solvent extracts of in vitro derived calli were examined for the antibacterial activity against the isolated human pathogens and the results are given in the table - 2. The maximum zone of inhibition 12 mm for Salmonella typhi, 13 mm for Klebsiella pneumoniae, 17 mm for Proteus vulgaris, 18 mm for Pseudomonas aeruginosa and B. subtilis and 10 mm for Staphylococcus aureus were observed. The ethanol extracts of leaf derived calli of O. basilicum illustrated the high degree of activity (5/6). The methods developed in this work make possible for the low volume and high potential production of active principles under in vitro condition in short duration with less amount of explants utilization.

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