The crude extract fractions of Pinus wallichiana leaves including n-hexane, ethyl acetate, chloroform, aqueous and residue were studied for antibacterial, antifungal, insecticidal and phytotoxic activities. All fractions exhibited no antibacterial activity against the tested bacterial strains. In case of antifungal bioassay, only n-hexane (F1) fraction showed minimum inhibition at the concentration of 25 μg/mL against Microsporum canis. The ethyl acetate fraction displayed activity against Tribolium castaneum, Rhyzopertha dominica and Callosobruchus analis. The other fraction showed activities against selected insects. In case of phytotoxic assay, all fractions showed concentration dependent activities. Then-hexane(F1), ethyl acetate (F2),chloroform (F3),water (F4) and residue (F5) fractions showed good phytotoxicity at 500μg/ml concentration and moderate activity at 50μg/ml. In short, the results showed that the plant is medicinally important.