Cicer arietinum is a potent medicinal plant in the Indian systems of medicine. Traditionally it is used as antibacterial, antifungal, antipyretic, ant idiarrhoeal etc. In the present study the hydroalcoholic extract and its acetone and methanol fractions of the root of C. arietinum were studied for their antibacterial activity by disc di ffusion method against different gram positive (Staphylococcus aureus and Bacillus subtilis) and g ram negative (Escherchia coli) bacteria. It was observed that the hydroalcoholic extract and it s acetone and methanol fraction showed significant activity against all the microorganisms tested here and the hydroalcoholic extract showed the highest activity(13 mm) against S. aureu s.