Argyreia speciosa is a potent medicinal plant in the Indian systems of medicine. Traditionally it is used as an antibacterial, antifungal, antipyretic, etc. In the present study the hydroalcoholic extract and its acetone, chloroform and methanol fractions of the root of A. speciosa were studied for their antibacterial activity by disc diffusion method against different gram positive (Staphylococcus aureus and Bacilus subtilis) and gram negative (Escherchia coli) bacteria. It was observed that the hydroalcoholic extract and its acetone, chloroform and methanol fraction showed significant activity against all the microorganisms tested here and the hydroalcoholic extract showed the highest activity (13 mm) against S. aureus.