Phenoxazines and its derivatives have shown diverse biological activities, but investigations on antibacterial activity are limited. In the present study antibacterial activities of the synthesized hexyl series compounds of phenoxazine 10-[6’-[N-Diethyl amino]hexyl]-2-chlorophenoxazine (10D), 10-[6’-[N-Bis(hydroxyethylamino)hexyl]-2-chloro phenoxazine (11D), 10-[6’-[N-Morpholino]hexyl]-2-chlorophenoxazine (12D), 10-[6’-[N-Piperidino]hexyl]-2- chlorophenoxazine (13D), 10-[6’-[N-Pyrrolidino]hexyl]- 2-chlorophenoxazine (14D), 10-[6’-[N-(β-hydroxyethyl) piperazino]-hexyl-2-chlorophenoxazine (15D) and 10-[6’-[N- Thiomorpholino]hexyl]-2-chlorophenoxazine (16D), were studied by the well-diffusion method. Bacteria such as Klebsialla sp., Bacillus sp., Proteus vulgaris, Salmonella sp., Shigella flexneri, Staphylococcus aureus, Streptococcus pneumoniae, and Vibrio cholerae were used to investigate the antibacterial activities. No antibacterial activity was observed with 12D and 16D while 15D exhibited antibacterial activity against a few tested bacteria. All other compounds (10D, 11D, 13D and 14D) studied exhibited significant antibacterial activities against all the bacteria screened.