Acacia arabica was investigated for preliminary phytochemical analysis and characterization by various instrumental techniques. Methanolic extracts of Acacia arabica seeds was very good antibacterial activity and also minimum inhibitory concentrating of different virus using HEL cell cultures HeLa cell cultures Vero cell cultures but MIC of Herpes simplex - 1 and 2, vaccinia virus, vesicular stomatitis and Herpes simplecx-1 (TK ACVI) were observed very good antiviral activity of Acacia arabica seeds DMSO extracts has good minimum cytotoxic concentration activity and also screening for various pharmaceutiocals activities. Such as anti oxidant and microbial activities.