Proton pump inhibitor is very effectively used in g astric disorder for reducing acid secretion. They v ery potent in nature and used only after therapy with histamine-2 (H2) receptor antagonists, known as H2 blockers, h ave been unsuccessful for symptoms of reflux. PPIs are inact ivated by exposure to gastric acid, due to rapid de gradation of these drugs in acidic and aqueous media, it is chal lenging to develop analytical method where in stabi lity of drug is least hampered. This review entitles different meth ods developed for determination of PPIs like UV-Spe ctroscopy, liquid Chromatography and LC-MS