Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 6

Analytical method development and validation of pioglitazone hydrochloride by RP-HPLC


An accurate high-performance liquid chromatography with UV-visible detection (HPLC-UV) method for the determination of pioglitazone hydrochloride [(± pyridinyl) ethoxy] phenyl] methyl]-2,4- ] is an oral ant diabetic agent belonging to the class of thiazolidinediones. Isocratic separation of Pioglitazone is carried out using a reversed-phase Intersil ODS C18 (150 mm × 4.6 mm, 5μm) column with mobile phase consisting of Ammonium acetate buffer with Acetonitrile and Glacial acetic acid in the ratio 50:50:1 (v/v) and quantified by UV detection at 269 nm with flow rate of 0.7ml/min. Specific and reproducible HPLC method has been developed and validated for quantitative determination in Pioglitazone Hydrochloride This method is accurate, precise, linear and can be used for routine analysis of Pioglitazone Hydrochloride.

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