The procedures of RP-HPLC-analysis of the tocols mixture and β-sitosterol and UV-Vis-analysis of carotenoids of Pumpkin - Cucurbita pepo - seed oil (25 %) in the presence of thymol (5 %) in the fat-based suppositories have been developed. Results of determination of γ- and α-tocopherols, β-sitosterol in suppositories was carried out by RPHPLC method according to United States Pharmacopeia with the aspect of linearity, accuracy and repeatability. It has been shown that the Pumpkin seed oil contains 6 mg % α-tocopherol, about 50 mg % γ-tocopherol and γ- tocotrienol mixture, 105-150 mg % β-sitosterol and 0,6 - 1,0 mg % carotenoids. Lutein and zeaxanthin in a ratio of (60:40) as the main carotenoids and cryptoxanthin, violaxanthin and other hydroxy- and epoxy-carotenoids as the minor components in the oil have been found. Accordingly, one suppository contains 240 µg tocols, 30-50 µg carotenoids and 630 µg β-sitosterol. The high content of β-sitosterol, thymol, γ-tocopherol and γ-tocotrienol mixture, as well as carotenoids allows the use of the medicine "Bioprost® " and Pumpkin seed oil as a pharmaceutical substance in other dosage forms for the treatment of lipid-associated diseases, benign prostatic hyperplasia and malignant neoplasms type myeloma.