GC-MS analysis was carried out to analyze the bioactive constituents in seeds of Mucuna pruriens Linn. Seven compounds were identified in ethyl acetate extract and eight ompounds were detected in ethanol extract by GC-MS analysis. This study reveals that Hexadecanoic acid, methyl ester, n- Hexadecanoic acid, 9, 12-Octadecadienoic acid, (Z,Z)- and Pyrrolidine,1-(1-oxo-7,10-hexadecadienyl)-were present on both extract. 1,2,3- Propanetriol,1- acetate, 9,12-Octadecadienoic acid, methylester, (E,E)-, and Deoxyspergualin were analyzed only on ethyl acetate extract. While Palmidrol, Hexadecanoic acid, 2-hydroxy-1-(hydroxyl methyl) ethyl ester, 9, 12-Octadecadienoic acid (Z,Z)-2-hydroxy-1-(hydroxymethyl) ethyl ester and 13-Docosenamide,(Z)- were found to be present only on ethanol extract. The presence of these bioactive constituents shows the pharmaceutical value of this plant.