Malathionis an organophosphorous pesticide that is widely used in agricultural and household applications to control pests. The purpose of this study was to evaluate the ameliorator property of vitamin C or/and curcumin againstmalathion-induced hepatotoxicity in male mice at the biochemical and histological levels. The mice were divided into nine groups. The first three groups were served as a control, while, the second two groups were treated with two different doses of malathion (Low and High doses). The third and fourth groups were treated with high and low dose of malathion with Curcumin.The fifth and sixth groups were treated with malathion (Low and High doses) combined with vitamin C. Meanwhile the eighth and ninth groups were treated with vitamin C and curcumin combined with malathion (Low and High doses).The experiment was continuous for a period of 30 days.Exposure to malathion at the twoexamined doses to mice led to an alteration of liver functions and lipid parameters. The effects of malathion on the biochemical parameters of mice were dose-dependent. Administration of curcumin or/and vitamin C to Mal-treated mice attenuates the toxicity of this compound, objectified by biochemical and histological improvement of liver. But, the alleviation is more pronounced with the both antioxidants. Thus, the synergistic effect of curcumin and vitamin C together is most powerful in reducing the toxicity induced by malathion and improving the hepatic and renal activities of mice