The emergence of the multiresistant bacteria responsible of nosocomial infections (NI) represented a real public health problem. These germs are the pathogenic opportunist ones such as the Methicillin-Resistant Staphylococcus aureus (MRSA) and Extended Spectrum Beta-Lactamases producing Gram-negative bacteria(ESBLpGN). The aim is to determine the effect of methanolic extract from the leaves of Marrubium vulgare against two Methicillin- Resistant Staphylococcus aureus (MRSA) and five Extended Spectrum Beta-Lactamases producing Gram-negative bacteria(ESBLpGN) (E.coli, P.vulgaris, k. pneumonia, Ps. aeruginosa and Ac baumanii) isolated from Urinary Nosocomial Infection and Sirgucal Site Infection, which was tested by both agar disc diffusion and microdilution methods. Marrubium vulgare extract more significantly inhibited the growth of Gram-positive (MRSA) from SSI than Extended Spectrum Beta-Lactamases producing Gram-negative bacteria (ESBL) from UIN. This study showed that Marrubium vulgare extract has a potent antibacterial activity against multiresistant pathogenic bacteria MRSA and ESBL producing Gram-negative responsible of NI. Further studies will be required to evaluate the practical values of therapeutic applications.