Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 7

Affinity chromatography mediated purification of hCG hormone

Abstract

Affinity chromatography by Carboxy methyl blue Sepharose was used to increase the purity and further the biological activity to more than 12000 IU/mg from 1.05mg of protein. The glycoprotein was then assessed for its conformational and functional ability in rats and could efficiently pronounce itself has a protein with increased purity