Rapid and accurate reverse phase high performance liquid chromatography method is described for determination of clopidogrel bisulphate from the pharmaceutical dosage form. It was observed that chromatopak peerless basic C18 (50 x 4.6 mm i.d.) with 3 μ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of buffer of ammonium acetate and acetonitrile. The detection was carried out at wavelength 220 nm. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution with the linear range as 50-150 μg/ ml. The method has been successfully used to assay of pharmaceutical dosage form i.e. tablets with good recoveries.