In recent years, the bio-sorption processes have been studied extensively using microbial biomass as biosorbent for heavy metal ions removal. In order to further study their biosorption potential, two algae Chlorococcum humicolum and Phormidium foveolarum have been studied under different initial metal concentrations. An experiment was conducted to study the rate of Cu accumulation in Chlorococcum humicolum and Phormidium foveolarum under laboratory condition. Algae isolated from sewage water treatment plant able to grow in copper concentration ranges from 0.1mgl-1 to 10mgl-1. Metal tolerant strain was obtained by repeated sub culturing. Tolerance of Chlorococcum humicolum to copper 1.5mgl-1 to 7 and Phormidium foveolarum 1.8 to 10mgl-1